Metagenomic Analysis Reveals Previously Undescribed Bat Coronavirus Strains in Eswatini

Ecohealth. 2021 Dec;18(4):421-428. doi: 10.1007/s10393-021-01567-3. Epub 2021 Dec 30.

Abstract

We investigated the prevalence of coronaviruses in 44 bats from four families in northeastern Eswatini using high-throughput sequencing of fecal samples. We found evidence of coronaviruses in 18% of the bats. We recovered full or near-full-length genomes from two bat species: Chaerephon pumilus and Afronycteris nana, as well as additional coronavirus genome fragments from C. pumilus, Epomophorus wahlbergi, Mops condylurus, and Scotophilus dinganii. All bats from which we detected coronaviruses were captured leaving buildings or near human settlements, demonstrating the importance of continued surveillance of coronaviruses in bats to better understand the prevalence, diversity, and potential risks for spillover.

Keywords: Chiroptera; alphacoronavirus; betacoronavirus; emerging infectious diseases; human–wildlife interface; zoonotic disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Chiroptera* / virology
  • Coronavirus Infections* / veterinary
  • Coronavirus* / genetics
  • Eswatini
  • Genetic Variation
  • Genome, Viral
  • Metagenomics*
  • Phylogeny