Three kinds of tumour-unique surface molecules on a human T-cell chronic lymphocytic leukaemia (T-CLL) detected by monoclonal antibodies

Scand J Immunol. 1987 Sep;26(3):237-46. doi: 10.1111/j.1365-3083.1987.tb02257.x.

Abstract

Mouse monoclonal antibodies recognizing cell surface molecules on tumour cells from a patient with a T-cell chronic lymphatic leukaemia (T-CLL) have been produced. Three different types of idiotype-like cell surface structures were identified. One molecule had a relative molecular weight (Mr) of 90,000 under non-reduced conditions and of 42,000 upon reduction, which corresponds well to the T-cell receptor for antigen (Ti). The two other molecules, which also behaved like unique tumour markers, have not previously been described, to our knowledge. One molecule was a monomer with an Mr of 74,000-80,000 when non-reduced and 80,000 upon reduction. The other idiotypic molecule was a dimer with a non-reduced Mr of 74,000-80,000, and 38,000 after reduction.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antibodies, Monoclonal / immunology*
  • Antibodies, Monoclonal / therapeutic use
  • Antigens, Neoplasm / analysis*
  • Antigens, Surface / analysis*
  • Digitonin / pharmacology
  • Humans
  • Immunoglobulin Variable Region / genetics
  • Leukemia, Lymphoid / immunology*
  • Lymphocyte Activation
  • Molecular Weight
  • Receptors, Antigen, T-Cell / analysis*
  • T-Lymphocytes / immunology

Substances

  • Antibodies, Monoclonal
  • Antigens, Neoplasm
  • Antigens, Surface
  • Immunoglobulin Variable Region
  • Receptors, Antigen, T-Cell
  • Digitonin