Analysis of mutagenic DNA repair in a thermoconditional mutant of Saccharomyces cerevisiae. III. Dose-response pattern of mutation induction in UV-irradiated rev2ts cells

Mol Gen Genet. 1986 Jan;202(1):68-74. doi: 10.1007/BF00330519.

Abstract

Recent studies regarding the influence of cycloheximide on the temperature-dependent increase in survival and mutation frequencies of a thermoconditional rev2 mutant lead to the suggestion that the REV2-coded mutagenic repair function is UV-inducible. In the present study we show that stationary-phase rev2ts cells are characterized by a biphasic linear-quadratic dose-dependence of mutation induction ("mutation kinetics") of ochre alleles at 23 degrees C (permissive temperature) but linear kinetics at the restrictive temperature of 36 degrees C. Mathematical analysis using a model based on Poisson statistics and a further mathematical procedure, the calculation of "apparent survival", support the assumption that the quadratic component of the reverse mutation kinetics investigated can be attributed to a UV-inducible component of mutagenic DNA repair controlled by the REV2 gene.

MeSH terms

  • DNA Repair*
  • Dose-Response Relationship, Radiation
  • Mathematics
  • Models, Genetic
  • Mutation*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / radiation effects*
  • Species Specificity
  • Ultraviolet Rays*