Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection

Bin Chen; Qin Tao; Fanglin Qiao; Yunjie Fei; Yuanjian Liu; Xiaohui Xiong; Songqin Liu

doi:10.1016/j.foodchem.2022.132404

Temporal sensing platform based on anodic dissolution of Ag and cathodic biocatalysis of oxygen reduction for Staphylococcus aureus detection

Food Chem. 2022 Jul 30:383:132404. doi: 10.1016/j.foodchem.2022.132404. Epub 2022 Feb 10.

Authors

Bin Chen¹, Qin Tao², Fanglin Qiao², Yunjie Fei², Yuanjian Liu³, Xiaohui Xiong², Songqin Liu⁴

Affiliations

¹ Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
² Coll Food Sci & Light Ind, Nanjing Tech University, Nanjing 211800, China.
³ Coll Food Sci & Light Ind, Nanjing Tech University, Nanjing 211800, China. Electronic address: lucias_cumt@163.com.
⁴ Jiangsu Engineering Laboratory of Smart Carbon-Rich Materials and Device, Laboratory of Environmental Medicine Engineering, Ministry of Education, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China. Electronic address: lsq@seu.edu.cn.

PMID: 35168048
DOI: 10.1016/j.foodchem.2022.132404

Abstract

An Ag@C hybrid bipolar electrode (BPE) sensing platform has been established for the temporal detection of Staphylococcus aureus (S. aureus) in food. Combining the advantages of anodic dissolution of Ag and cathodic biocatalysis of oxygen (O₂) reduction, this strategy showed an ultralow detection limit down to 10 CFU mL^-1. As the formation of Ag@C completely quenched the electrochemiluminescence (ECL) emission of luminol, the ECL emission recovery reflected the extent of anodic dissolution. Meanwhile, S. aureus catalyzed the electrochemical reduction of O₂ at the cathode, reducing the overpotential for cathodic O₂ reduction and thus increasing the rate of anodic electron loss, facilitating Ag dissolution and restoring the ECL emission of luminol. When a constant potential was applied, through monitoring the ECL recovery time before and after the incubation of S. aureus on the cathode, S. aureus could be quantified due to the slight difference of the conductivity.

Keywords: Anodic dissolution; Cathodic biocatalysis; O(2) reduction reaction; S. aureus; Temporal sensing.

MeSH terms

Biocatalysis
Biosensing Techniques*
Electrochemical Techniques
Electrodes
Luminescent Measurements
Luminol*
Oxygen
Solubility
Staphylococcus aureus

Substances

Luminol
Oxygen