Single-Molecule Imaging of mRNA Interactions with Stress Granules

Tatsuya Morisaki; Timothy J Stasevich

doi:10.1007/978-1-0716-1975-9_21

Single-Molecule Imaging of mRNA Interactions with Stress Granules

Methods Mol Biol. 2022:2428:349-360. doi: 10.1007/978-1-0716-1975-9_21.

Authors

Tatsuya Morisaki¹, Timothy J Stasevich^{2

3}

Affiliations

¹ Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA.
² Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA. Tim.Stasevich@colostate.edu.
³ World Research Hub Initiative, Institute of Innovative Research, Tokyo Institute of Technology, Yokohama, Kanagawa, Japan. Tim.Stasevich@colostate.edu.

Abstract

Single-molecule imaging in living cells enables the investigation of molecular dynamics and interactions underlying the physiology of a cell. We recently developed a method to visualize translation events at single-mRNA resolution in living cells. Here we describe how we apply this method to visualize mRNA interactions with stress granules in the context of translational activity during cell stress.

Keywords: MS2; RNA imaging; Single-molecule imaging; Stress granules; Translation.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, N.I.H., Extramural

MeSH terms

Cytoplasmic Granules / metabolism
Protein Biosynthesis
RNA, Messenger / genetics
RNA, Messenger / metabolism
Single Molecule Imaging* / methods
Stress Granules*

Substances

RNA, Messenger

Grants and funding

R35 GM119728/GM/NIGMS NIH HHS/United States