The cAMP signaling pathway regulates Epe1 protein levels and heterochromatin assembly

PLoS Genet. 2022 Feb 16;18(2):e1010049. doi: 10.1371/journal.pgen.1010049. eCollection 2022 Feb.

Abstract

The epigenetic landscape of a cell frequently changes in response to fluctuations in nutrient levels, but the mechanistic link is not well understood. In fission yeast, the JmjC domain protein Epe1 is critical for maintaining the heterochromatin landscape. While loss of Epe1 results in heterochromatin expansion, overexpression of Epe1 leads to defective heterochromatin. Through a genetic screen, we found that mutations in genes of the cAMP signaling pathway suppress the heterochromatin defects associated with Epe1 overexpression. We further demonstrated that the activation of Pka1, the downstream effector of cAMP signaling, is required for the efficient translation of epe1+ mRNA to maintain Epe1 overexpression. Moreover, inactivation of the cAMP-signaling pathway, either through genetic mutations or glucose deprivation, leads to the reduction of endogenous Epe1 and corresponding heterochromatin changes. These results reveal the mechanism by which the cAMP signaling pathway regulates heterochromatin landscape in fission yeast.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Heterochromatin / genetics
  • Heterochromatin / metabolism
  • Histones / genetics
  • Nuclear Proteins / genetics
  • Schizosaccharomyces pombe Proteins* / genetics
  • Schizosaccharomyces pombe Proteins* / metabolism
  • Schizosaccharomyces* / genetics
  • Schizosaccharomyces* / metabolism
  • Signal Transduction / genetics

Substances

  • Heterochromatin
  • Histones
  • Nuclear Proteins
  • Schizosaccharomyces pombe Proteins
  • epe1 protein, S pombe