Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine

N Okada; S Noguchi; S Nishimura; T Ohgi; T Goto; P F Crain; J A McCloskey

doi:10.1093/nar/5.7.2289

Structure determination of a nucleoside Q precursor isolated from E. coli tRNA: 7-(aminomethyl)-7-deazaguanosine

Nucleic Acids Res. 1978 Jul;5(7):2289-96. doi: 10.1093/nar/5.7.2289.

Authors

N Okada, S Noguchi, S Nishimura, T Ohgi, T Goto, P F Crain, J A McCloskey

Abstract

A precursor of modified nucleoside Q isolated from E. coli methyl-deficient tRNA was determined to be 7-(aminomethyl)-7-deazaguanosine. The structure was deduced by means of its chromatographic and electrophoretic mobilities, and UV and mass spectra, in addition to comparison with the synthesized authentic compound. The same molecule is also found in tRNA of an E. coli mutant selected for deficient synthesis of modified nucleosides.

MeSH terms

Chromatography, Thin Layer
Escherichia coli
Guanosine / analogs & derivatives*
Guanosine / biosynthesis
Mass Spectrometry
Nucleic Acid Precursors*
RNA, Bacterial
RNA, Transfer*

Substances

Nucleic Acid Precursors
RNA, Bacterial
Guanosine
RNA, Transfer