Increased thrombogenicity is associated with revascularization outcomes in patients with chronic limb-threatening ischemia

Abstract

Objectives: Clinically driven target lesion revascularization (CD-TLR) frequently occurs after endovascular therapy (EVT) in patients with chronic limb-threatening ischemia (CLTI). The total thrombus-formation analysis system (T-TAS) can quantitatively evaluate thrombogenicity. Therefore, we aimed to elucidate the association of the T-TAS parameters with CD-TLR.

Methods: We analyzed 34 patients with CLTI and 62 patients without CLTI who had undergone EVT. Blood samples collected on the day of EVT were used in the T-TAS to compute the thrombus formation area under the curve for the first 10 minutes for the platelet chip tested at a flow rate of 24 μL/min (PL₂₄-AUC₁₀) and area under the curve for the first 30 minutes for the atheroma chip tested at a flow rate of 10 μL/min (AR₁₀-AUC₃₀). After EVT, clinical follow-up was performed, and the presence of CD-TLR was assessed.

Results: During the follow-up period (median, 574 days), 10 patients (29%) in the CLTI group and 11 (18%) in the non-CLTI group had required CD-TLR. In the CLTI group, the patients with CD-TLR had had a higher AR₁₀-AUC₃₀ vs those without (median, 1694 [interquartile range, 1657-1799] vs median, 1561 [interquartile range, 1412-1697]; P = .01). In contrast, the PL₂₄-AUC₁₀ showed no significant differences when stratified by CD-TLR in either group. For the CLTI patients, multivariable Cox regression analysis using propensity score matching revealed that the AR₁₀-AUC₃₀ was an independent predictor of CD-TLR even after adjusting for baseline demographics, lesion characteristics, and anticoagulant use (hazard ratio, 2.04; 95% confidence interval, 1.18-3.88; P = .01; per 100-unit increase). In contrast, for those without CLTI, neither the AR₁₀-AUC₃₀ nor the PL₂₄-AUC₁₀ was significantly associated with CD-TLR. Receiver operating characteristics curve analysis identified an AR₁₀-AUC₃₀ level of 1646 as an optimal cutoff value to predict for CD-TLR (AUC, 0.85; sensitivity, 0.93; specificity, 0.56).

Conclusions: For patients with CLTI, but not for those without CLTI, the AR₁₀-AUC₃₀ showed potential to predict for CD-TLR. This finding suggests that hypercoagulability might play a predominant role in the progression of CLTI and that anticoagulant therapy might be useful in preventing revascularization.

Keywords: AR(10)-AUC(30); Chronic limb-threatening ischemia; T-TAS; Target lesion revascularization.