Functionalized Graphene Fiber Modified With MOF-Derived Rime-Like Hierarchical Nanozyme for Electrochemical Biosensing of H2O2 in Cancer Cells

Wei Huang; Yun Xu; Yimin Sun

doi:10.3389/fchem.2022.873187

Functionalized Graphene Fiber Modified With MOF-Derived Rime-Like Hierarchical Nanozyme for Electrochemical Biosensing of H₂O₂ in Cancer Cells

Front Chem. 2022 Mar 22:10:873187. doi: 10.3389/fchem.2022.873187. eCollection 2022.

Authors

Wei Huang^{1

2}, Yun Xu², Yimin Sun¹

Affiliations

¹ Hubei Key Laboratory of Plasma Chemistry and Advanced Materials, School of Materials Science and Engineering, Wuhan Institute of Technology, Wuhan, China.
² Key Laboratory of Material Chemistry for Energy Conversion and Storage, Ministry of Education, School of Chemistry and Chemical Engineering, Huazhong University of Science & Technology, Wuhan, China.

Abstract

The rational design and construction of high-performance flexible electrochemical sensors based on hierarchical nanostructure functionalized microelectrode systems are of vital importance for sensitive in situ and real-time detection of biomolecules released from living cells. Herein, we report a novel and facile strategy to synthesize a new kind of high-performance microelectrode functionalized by dual nanozyme composed of rime-like Cu₂(OH)₃NO₃ wrapped ZnO nanorods assembly [Cu₂(OH)₃NO₃@ZnO], and explore its practical application in electrochemical detection of hydrogen peroxide (H₂O₂) released from living cells. Benefiting from the merits of the unique hierarchical nanohybrid structure and high catalytic activities, the resultant Cu₂(OH)₃NO₃@ZnO-modified AGF microelectrode shows remarkable electrochemical sensing performance towards H₂O₂ with a low detection limit of 1 μM and a high sensitivity of 272 μA cm^-2 mM^-1, as well as good anti-interference capability, long-term stability, and reproducibility. These properties enabled the proposed microelectrode-based electrochemical platform to be applied for in situ amperometric tracking of H₂O₂ released from different types of human colon cells, thus demonstrating its great prospect as a sensitive cancer cell detection probe for the early diagnosis and management of various cancer diseases.

Keywords: flexible microelectrode; graphene fibers; hierarchical structure; in situ cancer cell detection; nanozymatic electrochemical sensor.