Effects of magnesium picolinate, zinc picolinate, and selenomethionine co-supplementation on reproductive hormones, and glucose and lipid metabolism-related protein expressions in male rats fed a high-fat diet

Kazim Sahin; Cemal Orhan; Osman Kucuk; Mehmet Tuzcu; Nurhan Sahin; Ibrahim H Ozercan; Sarah Sylla; Sara P Ojalvo; James R Komorowski

doi:10.1016/j.fochms.2022.100081

Effects of magnesium picolinate, zinc picolinate, and selenomethionine co-supplementation on reproductive hormones, and glucose and lipid metabolism-related protein expressions in male rats fed a high-fat diet

Food Chem (Oxf). 2022 Jan 27:4:100081. doi: 10.1016/j.fochms.2022.100081. eCollection 2022 Jul 30.

Authors

Kazim Sahin¹, Cemal Orhan¹, Osman Kucuk², Mehmet Tuzcu³, Nurhan Sahin¹, Ibrahim H Ozercan⁴, Sarah Sylla⁵, Sara P Ojalvo⁵, James R Komorowski⁵

Affiliations

¹ Department of Animal Nutrition, Faculty of Veterinary Medicine, Firat University, Elazig, Turkey.
² Department of Animal Nutrition, School of Veterinary Medicine, Erciyes University, 38039 Kayseri, Turkey.
³ Department of Biology, Faculty of Science, Firat University, Elazig, Turkey.
⁴ Department of Pathology, School of Medicine, Firat University, 23119 Elazig, Turkey.
⁵ Research and Development, Nutrition 21, Harrison, NY 10577, USA.

Abstract

This study aimed to examine the impacts of the magnesium picolinate (MgPic), zinc picolinate (ZnPic), and selenomethionine (SeMet) alone or as a combination on blood metabolites, oxidative enzymes, reproductive hormones, and glucose and lipid metabolism-related protein expressions in Wistar rats fed a high-fed diet (HFD). The rats were fed either a control, HFD, or HFD supplemented with a single (MgPic, ZnPic, SeMet) or two or three organic mineral combinations. Body weights, visceral fat, serum glucose, insulin, total cholesterol, triglycerides, leptin, malondialdehyde (MDA) concentrations as well as liver sterol regulatory element-binding protein-1c (SREBP-1c), liver X receptor alpha (LXRα), ATP citrate lyase (ACLY), fatty acid synthase (FAS), and nuclear factor kappa B (NF-κB) levels increased, while serum testosterone, follicle-stimulating hormone (FSH), luteinizing hormone (LH), sex hormone-binding globulin (SHBG), and insulin-like growth factor (IGF-1) concentrations along with liver nuclear factor erythroid 2-related factor 2 (Nrf2) levels declined in HFD rats (P < 0.05). Supplementing each organic mineral, but particularly the combination of HFD + MgPic + ZnPic + SeMet reversed the responses with various degrees. None of the organic elements alone or as a combination of two exerted a prominent effect on parameters measured. Although not additive or synergistic, the combination of all organic minerals added to HFD (HFD + MgPic + ZnPic + SeMet) provided the greatest responses.

Keywords: High-fat diet; Magnesium picolinate; Rat; Selenomethionine; Zinc picolinate.