Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis

Sho Tanosaki; Tomohiko Akiyama; Sayaka Kanaami; Jun Fujita; Minoru S H Ko; Keiichi Fukuda; Shugo Tohyama

doi:10.1016/j.xpro.2022.101360

Purification of cardiomyocytes and neurons derived from human pluripotent stem cells by inhibition of de novo fatty acid synthesis

STAR Protoc. 2022 Apr 28;3(2):101360. doi: 10.1016/j.xpro.2022.101360. eCollection 2022 Jun 17.

Authors

Sho Tanosaki¹, Tomohiko Akiyama², Sayaka Kanaami^{1

3}, Jun Fujita¹, Minoru S H Ko², Keiichi Fukuda¹, Shugo Tohyama¹

Affiliations

¹ Department of Cardiology, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.
² Department of Systems Medicine, Keio University School of Medicine, Shinjuku, Tokyo 160-8582, Japan.
³ Heartseed Inc., Shinjuku, Tokyo 160-0015, Japan.

Abstract

Here we describe a protocol to obtain highly pure cardiomyocytes and neurons from human induced pluripotent stem cells (hiPSCs) via metabolic selection processes. Compared to conventional purification protocols, this approach is easier to perform and scale up and more cost-efficient. The protocol can be applied to hiPSCs and human embryonic stem cells. For complete details on the use and execution of this protocol, please refer to Tohyama et al. (2016) and Tanosaki et al. (2020).

Keywords: Cell Differentiation; Cell culture; Cell isolation; Stem Cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Differentiation / physiology
Fatty Acids / pharmacology
Humans
Induced Pluripotent Stem Cells* / metabolism
Myocytes, Cardiac / physiology
Neurons
Pluripotent Stem Cells*

Substances

Fatty Acids