Serum and urine metabolomics analysis reveals the role of altered metabolites in patulin-induced nephrotoxicity

Food Res Int. 2022 Jun:156:111177. doi: 10.1016/j.foodres.2022.111177. Epub 2022 Mar 21.

Abstract

Various studies have identified the kidney as a target organ for patulin (PAT)-induced toxicity. However, detailed mechanistic insights into PAT-induced nephrotoxicity had not yet been done. Therefore, along with classical toxicological parameters, liquid chromatography-high resolution massed spectrometry (LC-HRMS) based metabolomics has been carried out to delineate the mechanism(s) of PAT-induced nephrotoxicity.An in vivo study was conducted using male Wistar rats, divided into three groups. PAT (25 µg/kg b.wt and 100 µg/kg b.wt) and, control were given through oral gavage, 5 days/week for 28 days. At the end of the experiment, changes in the mean body/ organ weight, food and water intake, expression of marker proteins of kidney injury, and histopathological changes were investigated. Furthermore, using LC-HRMS based metabolomics was performed on the serum and urine of PAT-exposed rats. The histopathological and toxicological analysis revealed a significant increase in glomerular mesangial cells, vacuolar degeneration, and cast deposition in the proximal convoluted tubules. The metabolomics showed metabolic perturbations in amino and fatty acid-related metabolic pathways in serum and urine of PAT-treated rats. In conclusion this study expands our understanding of PAT-induced metabolic alterations and its effects on renal function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Male
  • Mass Spectrometry
  • Metabolomics / methods
  • Patulin* / toxicity
  • Rats
  • Rats, Wistar
  • Urinalysis

Substances

  • Patulin