Lactobacillus plantarum ZY08 relieves chronic alcohol-induced hepatic steatosis and liver injury in mice via restoring intestinal flora homeostasis

Qinchao Ding; Feiwei Cao; Shanglei Lai; Hui Zhuge; Kaixin Chang; Teresa G Valencak; Jianxin Liu; Songtao Li; Daxi Ren

doi:10.1016/j.foodres.2022.111259

Lactobacillus plantarum ZY08 relieves chronic alcohol-induced hepatic steatosis and liver injury in mice via restoring intestinal flora homeostasis

Food Res Int. 2022 Jul:157:111259. doi: 10.1016/j.foodres.2022.111259. Epub 2022 Apr 16.

Authors

Qinchao Ding¹, Feiwei Cao², Shanglei Lai³, Hui Zhuge³, Kaixin Chang³, Teresa G Valencak⁴, Jianxin Liu⁵, Songtao Li⁶, Daxi Ren⁷

Affiliations

¹ Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China; School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, PR China.
² Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China; School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, PR China. Electronic address: caofeiwei@zju.edu.cn.
³ School of Life Science, Zhejiang Chinese Medical University, Hangzhou 310053, PR China.
⁴ Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: teresavalencak@zju.edu.cn.
⁵ Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: liujx@zju.edu.cn.
⁶ School of Public Health, Zhejiang Chinese Medical University, Hangzhou 310053, PR China. Electronic address: lisongtao@zcmu.edu.cn.
⁷ Institute of Dairy Science, College of Animal Sciences, Zhejiang University, Hangzhou 310058, PR China. Electronic address: dxren@zju.edu.cn.

PMID: 35761571
DOI: 10.1016/j.foodres.2022.111259

Abstract

This present study was designed to test the protective role of two Lactobacillus plantarum strains, E680 and ZY08, against alcoholic liver disease (ALD) in C57BL/6 mice. The ALD mouse model was established by exposing the mice to a Lieber-DeCarli ethanol liquid diet. The two probiotic strains (10⁹ cfu/day) were administered by oral gavage, respectively. Our data showed that L. plantarum ZY08, but not E680, intervention significantly mitigated alcohol-related hepatic steatosis, liver injury, intestinal barrier, and it alleviated plasma endotoxin (LPS) levels, and affected hepatic genes relating to lipid metabolism. Furthermore, Lactobacillus plantarum ZY08 effectively restored intestinal flora homeostasis via recovering flora abundance, including Blautia, Oscillibacter, Lachnoclostridium and Intestimonas, and consequently elevated intestinalshort-chain fatty acid (SCFA) content. More importantly, removing intestinal microorganisms through ABX gavage markedly abolished the beneficial aspects of Lactobacillus plantarum ZY08, indicating that the regulative role of Lactobacillus plantarum ZY08 contributed to its protective role against ALD. Overall, Lactobacillus plantarum ZY08 is a potential candidate for mitigating alcohol-induced hepatic steatosis and liver injury.

Keywords: Alcoholic liver disease; Hepatic steatosis; Intestinal barrier function; Intestinal flora homeostasis; Lactobacillus plantarum ZY08; Liver injury.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fatty Liver, Alcoholic* / prevention & control
Gastrointestinal Microbiome*
Homeostasis
Lactobacillus plantarum*
Liver Diseases, Alcoholic* / prevention & control
Mice
Mice, Inbred C57BL