Dual Mechanism of Action of Curcumin in Experimental Models of Multiple Sclerosis

Int J Mol Sci. 2022 Aug 4;23(15):8658. doi: 10.3390/ijms23158658.

Abstract

Background: Multiple sclerosis (MS) is characterized by a combination of inflammatory and demyelination processes in the spinal cord and brain. Conventional drugs generally target the autoimmune response, without any curative effect. For that reason, there is a great interest in identifying novel agents with anti-inflammatory and myelinating effects, to counter the inflammation and cell death distinctive of the disease.

Methods and results: An in vitro assay showed that curcumin (Cur) at 10 µM enhanced the proliferation of C8-D1A cells and modulated the production of Th1/Th2/Th17 cytokines in the cells stimulated by LPS. Furthermore, two in vivo pathophysiological experimental models were used to assess the effect of curcumin (100 mg/kg). The cuprizone model mimics the de/re-myelination aspect in MS, and the experimental autoimmune encephalomyelitis model (EAE) reflects immune-mediated events. We found that Cur alleviated the neurological symptomatology in EAE and modulated the expression of lymphocytes CD3 and CD4 in the spinal cord. Interestingly, Cur restored motor and behavioral deficiencies, as well as myelination, in demyelinated mice, as indicated by the higher index of luxol fast blue (LFB) and the myelin basic protein (MBP) intensity in the corpus callosum.

Conclusions: Curcumin is a potential therapeutic agent that can diminish the MS neuroimmune imbalance and demyelination through its anti-inflammatory and antioxidant effects.

Keywords: curcumin; demyelination; neuroinflammation.

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use
  • Curcumin* / pharmacology
  • Curcumin* / therapeutic use
  • Disease Models, Animal
  • Encephalomyelitis, Autoimmune, Experimental*
  • Mice
  • Mice, Inbred C57BL
  • Models, Theoretical
  • Multiple Sclerosis* / metabolism

Substances

  • Anti-Inflammatory Agents
  • Curcumin

Grants and funding

This research was funded by the Tunisian Ministry of High Education and Scientific Research.