Analysis of Cell Cycle by Flow Cytometry

Hamid Maadi; Mohammad Hasan Soheilifar; Zhixiang Wang

doi:10.1007/978-1-0716-2736-5_14

Analysis of Cell Cycle by Flow Cytometry

Methods Mol Biol. 2022:2579:183-195. doi: 10.1007/978-1-0716-2736-5_14.

Authors

Hamid Maadi¹, Mohammad Hasan Soheilifar², Zhixiang Wang³

Affiliations

¹ Department of Oncology, Cross Cancer Institute, University of Alberta, Edmonton, AB, Canada. hamid.maadi@gmail.com.
² Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran.
³ Department of Medical Genetics, University of Alberta, Edmonton, AB, Canada.

PMID: 36045207
DOI: 10.1007/978-1-0716-2736-5_14

Abstract

The cell cycle of a cell is tightly controlled by several regulators. Dysregulation of cell cycle can lead to uncontrolled cell division which is one of the main characteristics of cancer cells. DNA content of a cell is changed during the cell cycle progression and can be measured by flow cytometry. In this chapter, we aim to provide a detailed protocol on how to analyze the cell cycle using flow cytometry.

Keywords: Cell cycle; DNA contents; Flow cytometry; Propidium iodide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Cycle / physiology
Cell Division
Flow Cytometry* / methods
Propidium / metabolism

Substances

Propidium