Non-Invasive measurement of the cerebral metabolic rate of oxygen using MRI in rodents

Tobias C Wood; Diana Cash; Eilidh MacNicol; Camilla Simmons; Eugene Kim; David J Lythgoe; Fernando Zelaya; Federico Turkheimer

doi:10.12688/wellcomeopenres.16734.3

Non-Invasive measurement of the cerebral metabolic rate of oxygen using MRI in rodents

Wellcome Open Res. 2022 Jul 11:6:109. doi: 10.12688/wellcomeopenres.16734.3. eCollection 2021.

Authors

Tobias C Wood¹, Diana Cash¹, Eilidh MacNicol¹, Camilla Simmons¹, Eugene Kim¹, David J Lythgoe¹, Fernando Zelaya¹, Federico Turkheimer¹

Affiliation

¹ Department of Neuroimaging, Institute of Psychiatry, Psychology & Neuroscience, King's College London, SE5 8AF, UK.

Abstract

Malfunctions of oxygen metabolism are suspected to play a key role in a number of neurological and psychiatric disorders, but this hypothesis cannot be properly investigated without an in-vivo non-invasive measurement of brain oxygen consumption. We present a new way to measure the Cerebral Metabolic Rate of Oxygen (CMRO ₂) by combining two existing magnetic resonance imaging techniques, namely arterial spin-labelling and oxygen extraction fraction mapping. This method was validated by imaging rats under different anaesthetic regimes and was strongly correlated to glucose consumption measured by autoradiography.

Keywords: CBF; CMRO2; Glucose; MRI; Metabolism; OEF.

Associated data

figshare/10.6084/m9.figshare.14199035

Grants and funding

This study was funded by the Biotechnology and Biological Sciences Research Council (BB/N009088/1). TCW also received funding from the Wellcome/EPSRC Centre for Medical Engineering (Award number WT 203148). EM was supported by the Medical Research Council (MR/N013700/1) and King’s College London as a member of the MRC Doctoral Training Partnership in Biomedical Sciences.