Effect of different activation methods on the intratubular penetration of CaOH2 paste: Ex-vivo analysis by confocal laser scanning microscopy

Aust Endod J. 2023 Sep:49 Suppl 1:18-26. doi: 10.1111/aej.12685. Epub 2022 Sep 12.

Abstract

The aim of this study was to evaluate the maximum penetration depth (MPD) and penetration area (PA) of CaOH2 paste agitated by different methods and to measure the pH after performing these methods. Fifty-five mandibular premolars were divided into control, GL and 4 experimental groups, GEC, GXP, GEDDY and GI (n = 11), where the paste was agitated with Easy Clean, XP-endo Finisher, EDDY and Irrisonic, respectively. The canals were instrumented and irrigated with NaOCl. The paste was manipulated with propylene glycol and rhodamine B dye. Root sections were examined using confocal laser scanning microscopy. There was no difference between groups regarding PA (p > 0.05). At 5 mm, MPD was greater in GEC than in GL or GEDDY, and greater in GI than in GL (p < 0.05). At 2 mm, MPD was greater in GEC than in the other groups (p < 0.05). In conclusion, both Easy Clean and Irrisonic promoted deeper paste penetration and were associated with higher pH levels.

Keywords: calcium hydroxide; confocal laser scanning microscope; molar; root canal medicaments; root canal treatment.

MeSH terms

  • Bicuspid
  • Dental Pulp Cavity
  • Microscopy, Confocal
  • Propylene Glycol*
  • Root Canal Irrigants* / pharmacology
  • Root Canal Preparation / methods

Substances

  • Root Canal Irrigants
  • Propylene Glycol