Serologic versus molecular testing for screening for hepatitis C virus infection in patients with hematologic malignancies

Medicine (Baltimore). 2022 Sep 16;101(37):e30608. doi: 10.1097/MD.0000000000030608.

Abstract

Testing for antibody against hepatitis C virus (anti-HCV) is a low-cost diagnostic method worldwide; however, an optimal screening test for HCV in patients with cancer has not been established. We sought to identify an appropriate screening test for HCV infection in patients with hematologic malignancies and/or hematopoietic cell transplants (HCT). Patients in our center were simultaneously screened using serological (anti-HCV) and molecular (HCV RNA) assays (February 2019-November 2019). In total, 214 patients were enrolled in this study. Three patients (1.4%) were positive for anti-HCV, and 2 (0.9%) were positive for HCV RNA. The overall percentage agreement was 99.5% (95% CI: 97.4-99.9). There were no cases of seronegative HCV virus infection. The positive percentage agreement was 66.7% (95% CI: 20.8-93.9), and the negative percentage agreement was 100.0% (95% CI: 98.2-100.0). Cohen kappa coefficient was 0.80 (95% CI: 0.41-1.00, P < .0001). The diagnostic yield of screening for chronic HCV infection in patients with cancer is similar for serologic and molecular testing.

MeSH terms

  • Hematologic Neoplasms* / complications
  • Hepacivirus / genetics
  • Hepatitis C* / complications
  • Hepatitis C* / diagnosis
  • Humans
  • Molecular Diagnostic Techniques
  • RNA

Substances

  • RNA