Background: Platelet dysfunctions are shared by cardiovascular diseases and a wide range of inflammatory diseases.
Aims: To determine the ability of a new whole tomato-based food supplement (WTBFS) containing carotenoid and olive polyphenols to inhibit platelet aggregation.
Methods: Aggregation was evaluated in platelet-rich plasma using microtiter plates and a plate reader.
Results: Platelets treated with WTBFS showed a >70% reduction of 5 µM adenosine diphosphate (ADP)-induced platelet aggregation; at 10 µM of ADP, the inhibitory effect of WTBFS was reduced of about 50%. Similarly, 78% and 48% reduction were obtained using 5 µg/mL and 10 µg /mL of collagen as an agonist.
Conclusion: Since the compounds in WTBFS share the ability to inhibit STAT3, the inhibition of its signaling pathway may represent the mechanism underlying the antiplatelet activities. The activity of a lipophilic solution prepared from WTBS was in vitro tested on the platelet aggregation in response to ADP agonists and Collagen.
Keywords: antiplatelet activity; cardiovascular diseases; lycopene; platelet aggregation; tomato-based food supplement.