Expression and potential role of FOSB in glioma

Min Qi; Le-An Sun; Lan-Rong Zheng; Jia Zhang; Yan-Ling Han; Feng Wu; Jian Zhao; Wen-Hao Niu; Mao-Xing Fei; Xiao-Chun Jiang; Meng-Liang Zhou

doi:10.3389/fnmol.2022.972615

Expression and potential role of FOSB in glioma

Front Mol Neurosci. 2022 Oct 12:15:972615. doi: 10.3389/fnmol.2022.972615. eCollection 2022.

Authors

Min Qi¹, Le-An Sun², Lan-Rong Zheng³, Jia Zhang¹, Yan-Ling Han², Feng Wu⁴, Jian Zhao⁴, Wen-Hao Niu², Mao-Xing Fei², Xiao-Chun Jiang¹, Meng-Liang Zhou²

Affiliations

¹ The Translational Research Institute for Neurological Disorders of Wannan Medical College, Department of Neurosurgery, The First Affiliated Hospital of Wannan Medical College (Yijishan Hospital of Wannan Medical College), Wuhu, China.
² Department of Neurosurgery, Jinling Hospital, School of Medicine, Nanjing University, Nanjing, China.
³ Department of Pathology, Wannan Medical College, Wuhu, China.
⁴ Department of Anatomy, Wannan Medical College, Wuhu, China.

Abstract

Background: FOSB is reported to be an oncogene in a variety of tumors. However, the expression and role of FOSB in glioma remain obscure. In this study, we aimed to explore the expression of FOSB in glioma and its biological role in glioblastoma multiforme (GBM).

Methods: Western blot, immunohistochemical staining, and quantitative real-time polymerase chain reaction (RT-qPCR) were used to detect the expression of FOSB in clinical samples. FOSB was knocked down in cells to determine the effects of FOSB on the phenotypic changes of tumors by plate cloning, CCK-8 assay, and Transwell assay. Finally, subcutaneous tumorigenesis in nude mice was used to observe the tumorigenesis of glioma cell lines after the knockdown of the FOSB gene.

Results: FOSB expression was higher in glioma compared with normal brain tissue. After the downregulation of FOSB, the expression of cleaved caspase-3 increased. Plate cloning and CCK-8 experiments showed that the proliferation of glioma cell lines decreased. The Transwell assay demonstrated that the glioblastoma cell lines had lower migration ability after the knockdown of FOSB. Finally, the tumor volume of U87 glioma cells in group sh-FOSB was smaller than that in the control group. The TUNEL staining in vitro showed that the apoptosis of sh-FOSB glioma cells increased.

Conclusion: FOSB was highly expressed in glioma tissues. The viability of glioma cells decreased, and the ability of glioma cells to proliferate and migrate was reduced when FOSB was downregulated. Hence, FOSB may promote the development and migration of gliomas.

Keywords: FOSB; apoptosis; glioma; migration; proliferation.