Peripheral membrane proteins modulate stress tolerance by safeguarding cellulose synthases

Sci Adv. 2022 Nov 18;8(46):eabq6971. doi: 10.1126/sciadv.abq6971. Epub 2022 Nov 16.

Abstract

Controlled primary cell wall remodeling allows plant growth under stressful conditions, but how these changes are conveyed to adjust cellulose synthesis is not understood. Here, we identify the TETRATRICOPEPTIDE THIOREDOXIN-LIKE (TTL) proteins as new members of the cellulose synthase complex (CSC) and describe their unique and hitherto unknown dynamic association with the CSC under cellulose-deficient conditions. We find that TTLs are essential for maintaining cellulose synthesis under high-salinity conditions, establishing a stress-resilient cortical microtubule array, and stabilizing CSCs at the plasma membrane. To fulfill these functions, TTLs interact with CELLULOSE SYNTHASE 1 (CESA1) and engage with cortical microtubules to promote their polymerization. We propose that TTLs function as bridges connecting stress perception with dynamic regulation of cellulose biosynthesis at the plasma membrane.

MeSH terms

  • Arabidopsis Proteins* / genetics
  • Arabidopsis Proteins* / metabolism
  • Arabidopsis* / metabolism
  • Cell Membrane / metabolism
  • Cellulose / metabolism
  • Membrane Proteins / metabolism
  • Microtubules / metabolism

Substances

  • cellulose synthase
  • Arabidopsis Proteins
  • Cellulose
  • Membrane Proteins
  • TTL protein, Arabidopsis