Sequential histologic evolution of gallbladder inflammation in acute cholecystitis over the first 10 days after onset of symptoms

J Hepatobiliary Pancreat Sci. 2023 Jun;30(6):724-736. doi: 10.1002/jhbp.1274. Epub 2022 Dec 1.

Abstract

Background: The timing of cholecystectomy during acute cholecystitis (AC) is controversial, especially whether it is advisable to perform in patients with duration of symptoms between 3 and 10 days. The purpose of this study is to define clearly the sequential evolution of histological changes following symptoms onset to guide recommendations regarding timing of cholecystectomy.

Methods: We identified patients with AC (2005-2018) who had cholecystectomy within 10 days of symptom onset of a first attack of AC. Histologic features of gallbladder injury including cellular and exudative inflammatory response to injury were determined on blinded pathologic slides.

Results: One hundred and forty-nine patients were divided into three groups; early-who underwent cholecystectomy 1-3 days after symptom-onset, intermediate-4-6 days, and late-7-10 days. Key features of injury were necrosis and hemorrhage. A subgroup of patients in the early phase developed severe necrosis and hemorrhage of an extent associated with difficult cholecystectomy. Large spikes in extent of necrosis and hemorrhage occurred at 7-10 days. Major inflammatory responses to injury were eosinophilic and lymphocytic infiltration and early fibrosis.

Conclusions: Severe necrosis may develop rapidly and be present in the early period after symptom onset of AC. Cholecystectomy may be reasonable in some patients but by day 7-10, severe necrosis and hemorrhage may be expected to be present in most patients.

Keywords: acute cholecystitis; gallbladder hemorrhage; gallbladder myofibroblasts; gallbladder necrosis.

MeSH terms

  • Cholecystectomy
  • Cholecystectomy, Laparoscopic*
  • Cholecystitis* / pathology
  • Cholecystitis, Acute* / pathology
  • Cholecystitis, Acute* / surgery
  • Gallbladder / pathology
  • Gallbladder / surgery
  • Humans
  • Necrosis / pathology
  • Retrospective Studies