In vitro and in vivo models for monkeypox

Rafael Borges Rosa; Emilene Ferreira de Castro; Murilo Vieira da Silva; Denise Caroline Paiva Ferreira; Ana Carolina Gomes Jardim; Igor Andrade Santos; Mikaela Dos Santos Marinho; Flávia Batista Ferreira França; Lindomar José Pena

doi:10.1016/j.isci.2022.105702

In vitro and in vivo models for monkeypox

iScience. 2023 Jan 20;26(1):105702. doi: 10.1016/j.isci.2022.105702. Epub 2022 Dec 1.

Authors

Rafael Borges Rosa^{1

2}, Emilene Ferreira de Castro², Murilo Vieira da Silva², Denise Caroline Paiva Ferreira², Ana Carolina Gomes Jardim³, Igor Andrade Santos³, Mikaela Dos Santos Marinho³, Flávia Batista Ferreira França⁴, Lindomar José Pena¹

Affiliations

¹ Department of Virology and Experimental Therapy (LAVITE), Aggeu Magalhães Institute (IAM), Oswaldo Cruz Foundation (Fiocruz), Recife 50740-465, Brazil.
² Rodents Animal Facilities Complex, Federal University of Uberlândia (REBIR-UFU), Uberlândia 38400-902, Brazil.
³ Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405-302, Brazil.
⁴ Institute of Biomedical Sciences, Federal University of Uberlândia, Uberlândia 38405-318, Brazil.

Abstract

The emergence and rapid spread outside of monkeypox virus (MPXV) to non-endemic areas has led to another global health emergency in the midst of the COVID-19 pandemic. The scientific community has sought to rapidly develop in vitro and in vivo models that could be applied in research with MPXV. In vitro models include two-dimensional (2D) cultures of immortalized cell lines or primary cells and three-dimensional (3D) cultures. In vitro models are considered cost-effective and can be done in highly controlled conditions; however, they do not always resemble physiological conditions. In this way, several in vivo models are being characterized to meet the growing demand for new studies related to MPXV. In this review, we summarize the main MPXV models that have already been developed and discuss how they can contribute to advance the understanding of its pathogenesis, replication, and transmission, as well as identifying antivirals to treat infected patients.

Keywords: Virology.

Publication types

Review