Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens

Jiangshan Wang; Josiah Levi Davidson; Simerdeep Kaur; Andres A Dextre; Mohsen Ranjbaran; Mohamed S Kamel; Shreya Milind Athalye; Mohit S Verma

doi:10.3390/bios12121094

Paper-Based Biosensors for the Detection of Nucleic Acids from Pathogens

Biosensors (Basel). 2022 Nov 29;12(12):1094. doi: 10.3390/bios12121094.

Authors

Jiangshan Wang^{1

2}, Josiah Levi Davidson^{1

2}, Simerdeep Kaur^{1

2}, Andres A Dextre^{1

2}, Mohsen Ranjbaran^{1

2}, Mohamed S Kamel^{1

2

3}, Shreya Milind Athalye^{1

2}, Mohit S Verma^{1

2

4}

Affiliations

¹ Department of Agricultural and Biological Engineering, Purdue University, West Lafayette, IN 47907, USA.
² Birck Nanotechnology Center, Purdue University, West Lafayette, IN 47907, USA.
³ Department of Medicine and Infectious Diseases, Faculty of Veterinary Medicine, Cairo University, Giza 12211, Egypt.
⁴ Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.

Abstract

Paper-based biosensors are microfluidic analytical devices used for the detection of biochemical substances. The unique properties of paper-based biosensors, including low cost, portability, disposability, and ease of use, make them an excellent tool for point-of-care testing. Among all analyte detection methods, nucleic acid-based pathogen detection offers versatility due to the ease of nucleic acid synthesis. In a point-of-care testing context, the combination of nucleic acid detection and a paper-based platform allows for accurate detection. This review offers an overview of contemporary paper-based biosensors for detecting nucleic acids from pathogens. The methods and limitations of implementing an integrated portable paper-based platform are discussed. The review concludes with potential directions for future research in the development of paper-based biosensors.

Keywords: paper-based biosensors; pathogens; point-of-care testing.

Publication types

Review

MeSH terms

Biosensing Techniques* / methods
Lab-On-A-Chip Devices
Microfluidic Analytical Techniques*
Nucleic Acids*
Point-of-Care Systems
Point-of-Care Testing

Substances

Nucleic Acids

Grants and funding

UL1 TR002529/TR/NCATS NIH HHS/United States