The Meteorin-like cytokine is upregulated in grass carp after infection with Aeromonas hydrophila

Zhao Jia; Jianhua Feng; Gaoliang Yuan; Hehe Xiao; Huifeng Dang; Yanwei Zhang; Kangyong Chen; Jun Zou; Junya Wang

doi:10.1016/j.dci.2023.104632

The Meteorin-like cytokine is upregulated in grass carp after infection with Aeromonas hydrophila

Dev Comp Immunol. 2023 Apr:141:104632. doi: 10.1016/j.dci.2023.104632. Epub 2023 Jan 3.

Authors

Zhao Jia¹, Jianhua Feng¹, Gaoliang Yuan¹, Hehe Xiao¹, Huifeng Dang¹, Yanwei Zhang¹, Kangyong Chen¹, Jun Zou², Junya Wang³

Affiliations

¹ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
² Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China.
³ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: jywang@shou.edu.cn.

PMID: 36608897
DOI: 10.1016/j.dci.2023.104632

Abstract

Meteorin-like (Metrnl) is a novel immune regulatory factor or adipokine which is mainly produced by activated macrophages. In teleost fish, two homologs are present. In this study, monoclonal antibodies were prepared against recombinant grass carp (Ctenopharyngodon idella, Ci) Metrnl-a in mice and characterized by Western blotting, flow cytometry and immunofluorescent microscopy. In grass carp infected with Aeromonus hydrophila (A. hydrophila), the cells expressing CiMetrnl-a markedly increased in the gills, head kidney and intestine. In the inflamed intestine caused by A. hydrophila infection, the CiMetrnl-a producing cells were detected mainly in the mucosal layer of anterior, middle and posterior segments. Consistently, qRT-PCR analysis showed that the mRNA expression of CiMetrnl-a was markedly induced. Our results suggest that CiMetrnl-a is involved in regulating intestine inflammation caused by bacterial infection.

Keywords: Aeromonas hydrophila; Infection; Intestine; Meteorin-like; Monoclonal antibodies.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aeromonas hydrophila / physiology
Animals
Carps* / metabolism
Cytokines
Fish Diseases*
Fish Proteins* / metabolism
Gram-Negative Bacterial Infections*
Immunity, Innate
Mice

Substances

Cytokines
Fish Proteins