IL-27 suppresses spring viremia of carp virus replication in zebrafish

Yanjie Shi; Kangyong Chen; Xin Zhao; Yanan Lu; Wenji Huang; Jiahong Guo; Ning Ji; Zhao Jia; Hehe Xiao; Huifeng Dang; Jun Zou; Junya Wang

doi:10.1016/j.fsi.2023.108530

IL-27 suppresses spring viremia of carp virus replication in zebrafish

Fish Shellfish Immunol. 2023 Feb:133:108530. doi: 10.1016/j.fsi.2023.108530. Epub 2023 Jan 9.

Authors

Yanjie Shi¹, Kangyong Chen¹, Xin Zhao¹, Yanan Lu¹, Wenji Huang¹, Jiahong Guo¹, Ning Ji¹, Zhao Jia¹, Hehe Xiao¹, Huifeng Dang¹, Jun Zou², Junya Wang³

Affiliations

¹ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
² Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China. Electronic address: jzou@shou.edu.cn.
³ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: jywang@shou.edu.cn.

PMID: 36632914
DOI: 10.1016/j.fsi.2023.108530

Abstract

Interleukin (IL) 27 is a member of the IL-12 family and is a heterodimeric cytokine composed of IL-27A and Epstein-Barr virus-induced 3 (EBI3). It plays an important role in regulating inflammation and cancer progression. IL-27A not only functions by dimerizing with EBI3 but also acts alone. Here, we report that IL-27A and EBI3 suppress spring viremia of carp virus (SVCV) replication in zebrafish. Expression analysis reveals that il-27a and ebi3 were significantly upregulated in the ZF4 cells by SVCV and poly(I:C), and in the zebrafish caudal fin (ZFIN) cells overexpressed with SVCV genes. Interestingly, il-27a and ebi3 were not modulated by IFNφ1, indicating that they are not IFN stimulated genes (ISGs). Furthermore, overexpression of IL-27A and EBI3 alone inhibited SVCV replication in the EPC cells, but less potent than co-expression of IL-27A and EBI3. Intriguingly, IL-27A could not induce the expression of irf3, ifn, isg15 and mx1. Taken together, our results demonstrate that IL-27A and EBI3 activate innate antiviral response in an IFN independent manner in zebrafish.

Keywords: Antiviral response; EBI3; Interleukin-27A; SVCV; Zebrafish.

MeSH terms

Animals
Epstein-Barr Virus Infections
Fish Diseases*
Fish Proteins / genetics
Fish Proteins / metabolism
Herpesvirus 4, Human / metabolism
Interleukin-27* / genetics
Interleukins / genetics
Rhabdoviridae Infections* / veterinary
Rhabdoviridae* / physiology
Viremia
Virus Replication
Zebrafish* / genetics
Zebrafish* / metabolism

Substances

Fish Proteins
Interleukin-27
Interleukins

Supplementary concepts

Carp sprivivirus