Impact of conventional lipid-lowering therapy on circulating levels of PCSK9: protocol for a systematic review and meta-analysis of randomised controlled trials

BMJ Open. 2022 Sep 8;12(9):e061884. doi: 10.1136/bmjopen-2022-061884.

Abstract

Introduction: Conventional lipid-lowering agents, including statins, ezetimibe, fibrates, bile acid sequestrants, nicotinic acid, bempedoic acid and Omega-3, are essential to the management of dyslipidaemia. However, these agents have been shown to increase the level of plasma proprotein convertase subtilisin/kexin 9 (PCSK9), a serine protease associated with increased cardiovascular risk. This review aims to investigate the impact of commonly available conventional lipid-lowering agents on circulating PCSK9 levels and lipid profiles.

Methods and analysis: This protocol is conducted in accordance with the Preferred Reporting Items for Systematic Review and Meta-Analysis Protocols guidelines. A systematic search will be conducted in the following databases: MEDLINE, Cochrane Central Register of Controlled Trials (CENTRAL), EMBASE, Web of Science, SCOPUS and ScienceDirect. Additional information will be retrieved from clinical trial registries or from reference list searches. Published and peer-reviewed randomised controlled trials with adults receiving statin, ezetimibe, fibrate, bile acid sequestrant, nicotinic acid, bempedoic acid or Omega-3 monotherapy or in combination for at least 2 weeks, with availability of plasma PCSK9 at the beginning and end of treatment or the net changes in values, will be included. Study selection, data extraction and assessment of the risk of bias will be independently conducted by two investigators. Continuous data will be presented as a standardised mean difference with 95% confidence interval (CI) and dichotomous data as risk ratios with 95% CI. Subgroup analysis and sensitivity analysis will be performed when sufficient studies are included. Publication bias will be assessed with a funnel plot and Egger's test.

Ethics and dissemination: Ethics approval is not required as this review will only include data from published sources. The results will be published in a peer-reviewed journal.

Patient and public involvement: No patient or members of the general public are involved.

Prospero registration number: CRD42022297942.

Keywords: CLINICAL PHARMACOLOGY; Coronary heart disease; Lipid disorders.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Bile Acids and Salts
  • Ezetimibe / therapeutic use
  • Fatty Acids
  • Fibric Acids
  • Humans
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors* / therapeutic use
  • Hypolipidemic Agents / therapeutic use
  • Meta-Analysis as Topic
  • Niacin*
  • Proprotein Convertase 9
  • Proprotein Convertases
  • Review Literature as Topic
  • Subtilisin
  • Systematic Reviews as Topic

Substances

  • PCSK9 protein, human
  • Proprotein Convertase 9
  • 8-hydroxy-2,2,14,14-tetramethylpentadecanedioic acid
  • Niacin
  • Subtilisin
  • Hydroxymethylglutaryl-CoA Reductase Inhibitors
  • Fatty Acids
  • Hypolipidemic Agents
  • Proprotein Convertases
  • Ezetimibe
  • Fibric Acids
  • Bile Acids and Salts