High-grade neuroepithelial tumor with EP300::BCOR fusion and negative BCOR immunohistochemical expression: a case report

Brain Tumor Pathol. 2023 Apr;40(2):133-141. doi: 10.1007/s10014-023-00451-y. Epub 2023 Feb 22.

Abstract

In the World Health Organization tumor classification (fifth edition), central nervous system (CNS) tumors with BCOR internal tandem duplications have been recognized as a new tumor type. Some recent studies have reported CNS tumors with EP300::BCOR fusions, predominantly in children and young adults, expanding the spectrum of BCOR-altered CNS tumors. This study reports a new case of high-grade neuroepithelial tumor (HGNET) with an EP300::BCOR fusion in the occipital lobe of a 32-year-old female. The tumor displayed anaplastic ependymoma-like morphologies characterized by a relatively well-circumscribed solid growth with perivascular pseudorosettes and branching capillaries. Immunohistochemically, OLIG2 was focally positive and BCOR was negative. RNA sequencing revealed an EP300::BCOR fusion. The Deutsches Krebsforschungszentrum DNA methylation classifier (v12.5) classified the tumor as CNS tumor with BCOR/BCORL1 fusion. The t-distributed stochastic neighbor embedding analysis plotted the tumor close to the HGNET with BCOR alteration reference samples. BCOR/BCORL1-altered tumors should be included in the differential diagnosis of supratentorial CNS tumors with ependymoma-like histological features, especially when they lack ZFTA fusion or express OLIG2 even in the absence of BCOR expression. Analysis of published CNS tumors with BCOR/BCORL1 fusions revealed partly overlapping but not identical phenotypes. Further studies of additional cases are required to establish their classification.

Keywords: BCOR; EP300; Gene fusion; Neuroepithelial tumor.

Publication types

  • Case Reports

MeSH terms

  • Central Nervous System Neoplasms* / genetics
  • Central Nervous System Neoplasms* / metabolism
  • E1A-Associated p300 Protein / genetics
  • Ependymoma*
  • Female
  • Humans
  • Neoplasms, Neuroepithelial* / pathology
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins / metabolism
  • Repressor Proteins / genetics

Substances

  • Proto-Oncogene Proteins
  • BCOR protein, human
  • Repressor Proteins
  • EP300 protein, human
  • E1A-Associated p300 Protein