Optogenetic disruption of the prelimbic cortex alters long-term decision strategy but not valuation on a spatial delay discounting task

Abstract

Rats demonstrate a preference for smaller, immediate rewards over larger, delayed ones, a phenomenon known as delay-discounting (DD). Behavior arises from the interaction of multiple decision-making systems, and the medial prefrontal cortex (mPFC) has been identified as a central component in the mediation between these decision systems. To investigate the role of the prelimbic (PL) subregion of mPFC on decision strategy interaction, we compared two cohorts of rats (ChR2-opsin-expressing 'Active' and opsin-absent 'Control') on a spatial delay-discounting task while delivering in-vivo light stimulation into PL at the choice point of select trials. By analyzing the overall delay-adjustment along with deliberative and procedural behavioral strategy markers, our study revealed differences in the decision strategies used between the active and control animals despite both groups showing similar valuations. Control animals developed the expected shift from deliberative to procedural decision strategy on this task (indicated by reaching delay-stability, particularly during late-session laps); however, active-virus animals repeatedly over-adjusted around their preferred delay throughout the entire session, suggesting a significant deficit in procedural decision-making on this task. Active animals showed a significant decrease in proportion of vicarious trial and error events (VTE, a behavior correlated with deliberative processes) on delay adjustment laps relative to control animals. This points to a more nuanced role for VTE, not just in executing deliberation, but in shifting from deliberative to procedural processes. This opto-induced change in VTE was especially pronounced for late-session adjustment laps. We found no other session-by-session or lap-by-lap effects, leaving a particular role for PL in the long-term development of procedural strategies on this task.

Keywords: Delay discounting; Habit learning; Optogenetics; Procedural learning; Vicarious trial and error.