The expanded ISG12 family in zebrafish: ISG12.1 suppresses virus replication via targeting viral phosphoprotein

Jiahong Guo; Wenji Huang; Xin Zhao; Ning Ji; Kangyong Chen; Yanjie Shi; Jianhua Feng; Jun Zou; Junya Wang

doi:10.1016/j.dci.2023.104672

The expanded ISG12 family in zebrafish: ISG12.1 suppresses virus replication via targeting viral phosphoprotein

Dev Comp Immunol. 2023 May:142:104672. doi: 10.1016/j.dci.2023.104672. Epub 2023 Feb 22.

Authors

Jiahong Guo¹, Wenji Huang¹, Xin Zhao¹, Ning Ji¹, Kangyong Chen¹, Yanjie Shi¹, Jianhua Feng¹, Jun Zou², Junya Wang³

Affiliations

¹ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China.
² Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China; Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266200, China. Electronic address: jzou@shou.edu.cn.
³ Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of Education, Shanghai Ocean University, Shanghai, 201306, China; International Research Center for Marine Biosciences, Ministry of Science and Technology, Shanghai Ocean University, Shanghai, 201306, China; National Demonstration Center for Experimental Fisheries Science Education, Shanghai Ocean University, Shanghai, 201306, China. Electronic address: jywang@shou.edu.cn.

PMID: 36822549
DOI: 10.1016/j.dci.2023.104672

Abstract

In mammals, interferon (IFN)-stimulated genes (ISGs) play important roles in restricting the replication of viruses. However, the functions of many ISGs have not been investigated in fish. In this study, eight isg12 homologs (termed isg12.1-8) were identified in zebrafish and all contain a typical ISG12 family domain rich of hydrophobic amino acid residues. Isg12.1-7 were significantly induced in the ZF4 cells by poly(I:C) and IFNφ1, and in the kidney and spleen after infection with spring viremia of carp virus (SVCV). In the EPC cells, overexpression of isg12.1 inhibited SVCV replication. Further, it was found that zebrafish ISG12.1 interacted with SVCV phosphoprotein (SVCV-P) and promoted SVCV-P degradation which could be attenuated by 3-MA and CQ (autophagy inhibitors). Our results indicate that zebrafish ISG12.1 restricts viral replication by targeting viral phosphoprotein for degradation.

Keywords: Antiviral immunity; ISG12; Interferon; Phosphoprotein; SVCV.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Fish Diseases*
Mammals
Phosphoproteins / genetics
Rhabdoviridae Infections*
Rhabdoviridae*
Virus Replication
Zebrafish

Substances

Phosphoproteins

Supplementary concepts

Carp sprivivirus