Human small intestine contains 2 functionally distinct regulatory T-cell subsets

Sudhir Kumar Chauhan; Raquel Bartolomé Casado; Ole J B Landsverk; Hanna Johannessen; Danh Phung; Hogne Røed Nilsen; Frank Sætre; Jørgen Jahnsen; Rune Horneland; Sheraz Yaqub; Einar Martin Aandahl; Knut E A Lundin; Espen S Bækkevold; Frode L Jahnsen

doi:10.1016/j.jaci.2023.02.030

Human small intestine contains 2 functionally distinct regulatory T-cell subsets

J Allergy Clin Immunol. 2023 Jul;152(1):278-289.e6. doi: 10.1016/j.jaci.2023.02.030. Epub 2023 Mar 8.

Affiliations

¹ Department of Pathology, Oslo University Hospital-Rikshospitalet, Oslo, Norway; Department of Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, Oslo, Norway. Electronic address: sudhir.chauhan.kumar@rr-research.no.
² Department of Pathology, Oslo University Hospital-Rikshospitalet, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
³ Department of Pathology, Oslo University Hospital-Rikshospitalet, Oslo, Norway.
⁴ Department of Pathology, Oslo University Hospital-Rikshospitalet, Oslo, Norway; Department of Gastrointestinal and Pediatric Surgery, Oslo University Hospital-Rikshospitalet, Oslo, Norway.
⁵ Institute of Clinical Medicine, University of Oslo, Oslo, Norway; Department of Gastroenterology, Akershus University Hospital, Lørenskog, Norway.
⁶ Department of Transplantation Medicine, Section for Transplant Surgery, Oslo University Hospital-Rikshospitalet, Oslo, Norway.
⁷ Department of Gastrointestinal Surgery, Oslo University Hospital-Rikshospitalet, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
⁸ Department of Gastroenterology, Oslo University Hospital-Rikshospitalet, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway.
⁹ Department of Pathology, Oslo University Hospital-Rikshospitalet, Oslo, Norway; Institute of Clinical Medicine, University of Oslo, Oslo, Norway. Electronic address: f.l.jahnsen@medisin.uio.no.

PMID: 36893861
DOI: 10.1016/j.jaci.2023.02.030

Abstract

Background: Regulatory T (Treg) CD4 cells in mouse gut are mainly specific for intestinal antigens and play an important role in the suppression of immune responses against harmless dietary antigens and members of the microbiota. However, information about the phenotype and function of Treg cells in the human gut is limited.

Objective: We performed a detailed characterization of Foxp3⁺ CD4 Treg cells in human normal small intestine (SI) as well as from transplanted duodenum and celiac disease lesions.

Methods: Treg cells and conventional CD4 T cells derived from SI were subjected to extensive immunophenotyping and their suppressive activity and ability to produce cytokines assessed.

Results: SI Foxp3⁺ CD4 T cells were CD45RA^-CD127^-CTLA-4⁺ and suppressed proliferation of autologous T cells. Approximately 60% of Treg cells expressed the transcription factor Helios. When stimulated, Helios-negative Treg cells produced IL-17, IFN-γ, and IL-10, whereas Helios-positive Treg cells produced very low levels of these cytokines. By sampling mucosal tissue from transplanted human duodenum, we demonstrated that donor Helios-negative Treg cells persisted for at least 1 year after transplantation. In normal SI, Foxp3⁺ Treg cells constituted only 2% of all CD4 T cells, while in active celiac disease, both Helios-negative and Helios-positive subsets expanded 5- to 10-fold.

Conclusion: The SI contains 2 subsets of Treg cells with different phenotypes and functional capacities. Both subsets are scarce in healthy gut but increase dramatically in active celiac disease.

Keywords: CD4 T cells; Foxp3; Helios; Regulatory T (Treg) cells; celiac disease; human small intestine; transplantation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Celiac Disease*
Cytokines
Forkhead Transcription Factors
Humans
Intestine, Small
Mice
T-Lymphocyte Subsets
T-Lymphocytes, Regulatory*

Substances

Cytokines
Forkhead Transcription Factors