Effect of transduced mesenchymal stem cells with IL-10 gene on control of allergic asthma

Allergol Immunopathol (Madr). 2023 Mar 1;51(2):45-51. doi: 10.15586/aei.v51i2.789. eCollection 2023.

Abstract

Asthma is an important pulmonary disease associated with T helper lymphocyte (Th)2 dominant immune response, which can initiate allergic and inflammatory reactions. Interleukin (IL)-10 is the main immune suppressor cytokine, and mesenchymal stem cells (MSCs) have an immune-modulatory potential that can be transduced with the expression of the IL-10 gene to control pathophysiology of allergic asthma. Bone marrow's MSCs were isolated and transduced with the expression vector that contains the expressible IL-10 gene. Then, allergic asthma mouse model was produced and treated with manipulated MSCs. Methacholine challenge test; measurement of IL-4, IL-5, IL-8, IL-13, IL-25, and IL-33; and total and ovalbumin (OVA)-specific immunoglobulin (Ig)E levels were done. Hyperplasia of the goblet cell, secretion of mucus, and peribronchiolar and perivascular eosinophilic inflammation were evaluated in lung pathological sections. IL-25, IL-33, and total IgE levels; AHR; eosinophilic inflammation; hyperplasia of the goblet cell; and secretion of mucus could be controlled in M, MV, and MV-10 groups, and the control in the MV-10 group was strong compared to M and MV groups. MSCs have immune-modulatory capacity that can control allergic asthma pathophysiology, and this effect can be strengthened and reinforced by the expression of IL-10 gene.

Keywords: Allergy; Cell therapy; Cytokine; Gene therapy; Inflammation.

MeSH terms

  • Animals
  • Asthma* / genetics
  • Asthma* / therapy
  • Bronchoalveolar Lavage Fluid
  • Cytokines
  • Disease Models, Animal
  • Eosinophilia*
  • Hyperplasia / pathology
  • Immunoglobulin E
  • Inflammation
  • Interleukin-10 / genetics
  • Interleukin-33
  • Lung / pathology
  • Mesenchymal Stem Cells*
  • Mice
  • Mice, Inbred BALB C
  • Ovalbumin

Substances

  • Cytokines
  • Immunoglobulin E
  • Interleukin-10
  • Interleukin-33
  • Ovalbumin
  • IL10 protein, mouse