Selectivity of Insecticides to a Pupal Parasitoid, Trichospilus diatraeae (Hymenoptera: Eulophidae), of Soybean Caterpillars

Insects. 2023 Feb 22;14(3):217. doi: 10.3390/insects14030217.

Abstract

Selectivity is an important aspect of modern insecticides to be able to target pests whilst maintaining beneficial entomofauna in the crop. The present objective was to assess the selectivity of different insecticides for the pupal parasitoid of soybean caterpillars, i.e., Trichospilus diatraeae Cherian & Margabandhu, 1942 (Hymenoptera: Eulophidae). Acephate, azadirachtin, Bacillus thuringiensis (Bt), deltamethrin, lufenuron, teflubenzuron and thiamethoxam + lambda-cyhalothrin at the highest recommended concentrations for the soybean looper Chrysodeixis includens (Walker, [1858]) (Lepidoptera: Noctuidae), as well as water in the control, were used against the pupal parasitoid T. diatraeae. The insecticides and the control were sprayed on the soybean leaves, which were left to dry naturally and placed in cages with T. diatraeae females in each one. Survival data were submitted to analysis of variance (ANOVA) and the means were compared using Tukey's HSD test (α = 0.05). Survival curves were plotted according to the Kaplan-Meier method, and the pairs of curves were compared using the log-rank test at 5% probability. The insecticides azadirachtin, Bt, lufenuron and teflubenzuron did not affect T. diatraeae survival, while deltamethrin and thiamethoxam + lambda-cyhalothrin presented low toxicity and acephate was highly toxic, causing 100% mortality in the parasitoid. Azadirachtin, Bt, lufenuron and teflubenzuron are selective for T. diatraeae and could be used in IPM programs.

Keywords: Eulophidae; IPM; mortality; parasitoid; pesticide; selectivity.

Grants and funding

This work was supported by Associação Sul-Mato-Grossense de Produtores e Consumidores de Florestas Plantadas (Reflore-MS); Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq 304055/2019-0; 437490/2018-1); Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES); Fundação de Apoio ao Desenvolvimento da Educação, Ciência e Tecnologia do Estado de Mato Grosso do Sul (FUNDECT); JB Biotecnologia e Grupo Vittia and “Programa Cooperativo sobre Proteção Florestal (PROTEF) do Instituto de Pesquisas e Estudos Florestais (IPEF)”.