Development of a reproducible small intestinal microbiota model and its integration into the SHIME®-system, a dynamic in vitro gut model

Stef Deyaert; Frédéric Moens; Walter Pirovano; Bartholomeus van den Bogert; Eline Suzanne Klaassens; Massimo Marzorati; Tom Van de Wiele; Michiel Kleerebezem; Pieter Van den Abbeele

doi:10.3389/fmicb.2022.1054061

Development of a reproducible small intestinal microbiota model and its integration into the SHIME^®-system, a dynamic in vitro gut model

Front Microbiol. 2023 Mar 17:13:1054061. doi: 10.3389/fmicb.2022.1054061. eCollection 2022.

Affiliations

¹ ProDigest BV, Gent, Belgium.
² Baseclear, Leiden, Netherlands.
³ Center of Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent University, Gent, Belgium.
⁴ Department of Animal Sciences, Wageningen University, Wageningen, Netherlands.

Abstract

The human gastrointestinal tract consists of different regions, each characterized by a distinct physiology, anatomy, and microbial community. While the colonic microbiota has received a lot of attention in recent research projects, little is known about the small intestinal microbiota and its interactions with ingested compounds, primarily due to the inaccessibility of this region in vivo. This study therefore aimed to develop and validate a dynamic, long-term simulation of the ileal microbiota using the SHIME^®-technology. Essential parameters were identified and optimized from a screening experiment testing different inoculation strategies, nutritional media, and environmental parameters over an 18-day period. Subjecting a synthetic bacterial consortium to the selected conditions resulted in a stable microbiota that was representative in terms of abundance [8.81 ± 0.12 log (cells/ml)], composition and function. Indeed, the observed community mainly consisted of the genera Streptococcus, Veillonella, Enterococcus, Lactobacillus, and Clostridium (qPCR and 16S rRNA gene targeted Illumina sequencing), while nutrient administration boosted lactate production followed by cross-feeding interactions towards acetate and propionate. Furthermore, similarly as in vivo, bile salts were only partially deconjugated and only marginally converted into secondary bile salts. After confirming reproducibility of the small intestinal microbiota model, it was integrated into the established M-SHIME® where it further increased the compositional relevance of the colonic community. This long-term in vitro model provides a representative simulation of the ileal bacterial community, facilitating research of the ileum microbiota dynamics and activity when, for example, supplemented with microbial or diet components. Furthermore, integration of this present in vitro simulation increases the biological relevance of the current M-SHIME® technology.

Keywords: ileum; in vitro model; microbiota; short-chain fatty acid; simulator of the human intestinal microbial ecosystem; small intestine.