The Potential Use of Mitochondrial Extracellular Vesicles as Biomarkers or Therapeutical Tools

Int J Mol Sci. 2023 Apr 10;24(8):7005. doi: 10.3390/ijms24087005.

Abstract

The mitochondria play a crucial role in cellular metabolism, reactive oxygen species (ROS) production, and apoptosis. Aberrant mitochondria can cause severe damage to the cells, which have established a tight quality control for the mitochondria. This process avoids the accumulation of damaged mitochondria and can lead to the release of mitochondrial constituents to the extracellular milieu through mitochondrial extracellular vesicles (MitoEVs). These MitoEVs carry mtDNA, rRNA, tRNA, and protein complexes of the respiratory chain, and the largest MitoEVs can even transport whole mitochondria. Macrophages ultimately engulf these MitoEVs to undergo outsourced mitophagy. Recently, it has been reported that MitoEVs can also contain healthy mitochondria, whose function seems to be the rescue of stressed cells by restoring the loss of mitochondrial function. This mitochondrial transfer has opened the field of their use as potential disease biomarkers and therapeutic tools. This review describes this new EVs-mediated transfer of the mitochondria and the current application of MitoEVs in the clinical environment.

Keywords: MitoEVs; biomarker; extracellular vesicles; mitochondria; therapy.

Publication types

  • Review

MeSH terms

  • Biomarkers / metabolism
  • DNA, Mitochondrial / genetics
  • Extracellular Vesicles* / metabolism
  • Mitochondria* / metabolism
  • Reactive Oxygen Species / metabolism

Substances

  • DNA, Mitochondrial
  • Reactive Oxygen Species
  • Biomarkers

Grants and funding

This work was supported by the following grants: Grant PID2020–113839RB-I00 funded by MCIN/AEI/10.13039/501100011033, PCIN-2017–117 of the Ministry of Economy and Competitiveness, and the EU Joint Programming Initiative “A Healthy Diet for a Healthy Life” (JPI HDHL INTIMIC-085) to C.B., and CIGE/2021/134 from Conselleria de Innovación, Universidades, Ciencia y Sociedad Digital to C.M.-B. Part of the equipment employed in this work was funded by Generalitat Valenciana and co-financed with ERDF funds (OP ERDF of Comunitat Valenciana 2014–2020).