Protocol to generate cardiac pericytes from human induced pluripotent stem cells

Mengcheng Shen; Shane Rui Zhao; Yaser Khokhar; Li Li; Yang Zhou; Chun Liu; Joseph C Wu

doi:10.1016/j.xpro.2023.102256

Protocol to generate cardiac pericytes from human induced pluripotent stem cells

STAR Protoc. 2023 Apr 27;4(2):102256. doi: 10.1016/j.xpro.2023.102256. Online ahead of print.

Authors

Mengcheng Shen¹, Shane Rui Zhao², Yaser Khokhar², Li Li², Yang Zhou², Chun Liu³, Joseph C Wu⁴

Affiliations

¹ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: mcshen@stanford.edu.
² Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA.
³ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Greenstone Biosciences, Palo Alto, CA 94304, USA.
⁴ Stanford Cardiovascular Institute, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Medicine, Division of Cardiovascular Medicine, Stanford University School of Medicine, Stanford, CA 94305, USA; Greenstone Biosciences, Palo Alto, CA 94304, USA; Department of Radiology, Stanford University School of Medicine, Stanford, CA 94305, USA. Electronic address: joewu@stanford.edu.

Abstract

Cardiac pericytes are a critical yet enigmatic cell type within the coronary microvasculature. Since primary human cardiac pericytes are not readily accessible, we present a protocol to generate them from human induced pluripotent stem cells (hiPSCs). Our protocol involves several steps, including the generation of intermediate cell types such as mid-primitive streak, lateral plate mesoderm, splanchnic mesoderm, septum transversum, and epicardium, before deriving cardiac pericytes. With hiPSC-derived cardiac pericytes, researchers can decipher the mechanisms underlying coronary microvascular dysfunction. For complete details on the use and execution of this protocol, please refer to Shen et al.¹.

Keywords: Cell Differentiation; Developmental Biology; Stem Cells.

Abstract

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