Integrated FT-ICR MS and metabolome reveals diatom-derived organic matter by bacterial transformation under warming and acidification

Yang Liu; Chao Ma; Jun Sun

doi:10.1016/j.isci.2023.106812

Integrated FT-ICR MS and metabolome reveals diatom-derived organic matter by bacterial transformation under warming and acidification

iScience. 2023 May 4;26(6):106812. doi: 10.1016/j.isci.2023.106812. eCollection 2023 Jun 16.

Authors

Yang Liu^{1

2

3}, Chao Ma⁴, Jun Sun^{1

2}

Affiliations

¹ Institute for Advance Marine Research, China University of Geosciences, Guangzhou 511462, China.
² State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, China.
³ Institute of Marine Science and Technology, Shandong University, Qingdao 266237, China.
⁴ Institute of Surface-Earth System Science, School of Earth System Science, Tianjin University, Tianjin 300072, China.

Abstract

Bacterial transformation and processing of diatom-derived organic matter (OM) is extremely important for the cycling of production and energy in marine ecosystems; this process contributes to the production of microbial food webs. In this study, a cultivable bacterium (Roseobacter sp. SD-R1) from the marine diatom Skeletonema dohrnii were isolated and identified. A combined Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS)/untargeted metabolomics approach was used to synthesize the results of bacterial transformation with dissolved OM (DOM) and lysate OM (LOM) under warming and acidification through laboratory experiments. Roseobacter sp. SD-R1 had different preferences for the conversion of molecules in S. dohrnii-derived DOM and LOM treatments. The effects of warming and acidification contribute to the increased number and complexity of molecules of carbon, hydrogen, oxygen, nitrogen, and sulfur after the bacterial transformation of OM. The chemical complexity generated by bacterial metabolism provides new insights into the mechanisms that shape OM complexity.

Keywords: Earth sciences; Global change; Marine geochemistry.