Extrusion of casein and whey protein isolate enhances anti-hardening and performance in high-protein nutrition bars

Kaili Wang; Xu Zhao; Munkh-Amgalan Gantumur; Jinzhe Li; Yuxuan Huang; Narantuya Sukhbaatar; Tian Bo; Zhanmei Jiang

doi:10.1016/j.fochx.2023.100719

Extrusion of casein and whey protein isolate enhances anti-hardening and performance in high-protein nutrition bars

Food Chem X. 2023 May 22:18:100719. doi: 10.1016/j.fochx.2023.100719. eCollection 2023 Jun 30.

Authors

Kaili Wang¹, Xu Zhao¹, Munkh-Amgalan Gantumur¹, Jinzhe Li¹, Yuxuan Huang¹, Narantuya Sukhbaatar², Tian Bo¹, Zhanmei Jiang¹

Affiliations

¹ Key Laboratory of Dairy Science (Northeast Agricultural University), Ministry of Education, College of Food Science, Northeast Agricultural University, Harbin 150030, PR China.
² School of Industrial Technology, Mongolian University of Science and Technology, 14191, Baga toiruu 34, Sukhbaatar District, Ulaanbaatar, Mongolia.

Abstract

Model high-protein nutrition bars (HPNBs) were formulated by incorporating whey protein isolate (WPI) and casein (CN) at various extrusion temperatures (50, 75, 100, 125, and 150 °C) with a protein content of 45 g per 100 g. The free sulfhydryl groups, amino groups, hardness, and microstructures of HPNBs were analyzed periodically at 37 °C over a storage period of 45 days. Specifically, sulfhydryl group, amino group and surface hydrophobicity of extruded whey protein isolate (WPE) and extruded casein (CE) were significantly reduced (P < 0.05) compared to those of unextruded protein. HPNBs formulated with WPE (HWPE) and CE (HWCE) exhibited a slower hardening rate compared to those formulated with unmodified protein. Moreover, the color difference, hardness and sensory score of HPNBs after 45 days of storage were used as indicators, and the results of the TOPSIS multiple index analysis indicated that HPNB formulated with WPI extruded at 150 °C possessed the best quality characteristics.

Keywords: Casein (CN); Extrusion; High-protein nutrition bars (HPNBs); Whey protein isolate (WPI).