The value of microbiology rapid on-site evaluation of sepsis caused by pulmonary infection

Eur Rev Med Pharmacol Sci. 2023 Jun;27(12):5862-5868. doi: 10.26355/eurrev_202306_32825.

Abstract

Objective: This study aims to evaluate the value of microbial rapid on-site evaluation (M-ROSE) of sepsis, and septic shock caused by pulmonary infection.

Patients and methods: Thirty-six patients with sepsis and septic shock due to hospital-acquired pneumonia were analyzed. Accuracy and time were compared with M-ROSE, traditional culture, and next-generation sequencing (NGS).

Results: A total of 48 strains of bacteria and 8 strains of fungi were detected by bronchoscopy in 36 patients. The accuracy rate of bacteria and fungi was 95.8% and 100%, respectively. M-ROSE took an average of 0.34±0.01 hours, much faster than NGS (22h±0.01 h, p<0.0001) and traditional culture time (67.50±0.91 h, p<0.0001).

Conclusions: M-ROSE may quickly identify common bacteria and fungi, so it may be a useful method for the etiological diagnosis of sepsis and septic shock caused by pulmonary infection.

MeSH terms

  • Bacteria
  • Fungi
  • Humans
  • Pneumonia* / diagnosis
  • Rapid On-site Evaluation
  • Retrospective Studies
  • Sepsis* / diagnosis
  • Sepsis* / microbiology
  • Shock, Septic* / microbiology