Prevalence of Glu323Lys Mutation of the TIGR/MYOC Gene and Risk Factors amongst Primary Open-angle Glaucoma Patients in Ouagadougou, Burkina Faso

Lassina Traoré; Jérôme Sanou; Bélélé S Bakyono; Abdou A Zoure; Théodora M Zohoncon; Hermann K Sombié; Albert T Yonli; Guertrude Meda-Hien; Ezechiel B Tibiri; Florencia W Djigma; Jacques Simpore

doi:10.5005/jp-journals-10078-1403

Prevalence of Glu323Lys Mutation of the TIGR/MYOC Gene and Risk Factors amongst Primary Open-angle Glaucoma Patients in Ouagadougou, Burkina Faso

J Curr Glaucoma Pract. 2023 Apr-Jun;17(2):79-84. doi: 10.5005/jp-journals-10078-1403.

Affiliations

¹ Department of Molecular Biology, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou 01, Burkina Faso; Department of Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou 03, Burkina Faso.
² Department of Ophthalmology, Joseph KI ZERBO University, CHU Yalgado OUEDRAOGO, Ouagadougou 03, Burkina Faso.
³ Department of Molecular Biology, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou 01, Burkina Faso; Department of Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou 03, Burkina Faso; Department of Biomedical and Public Health, Institute for Research in Health Sciences (IRSS/CNRST), Ouaga 03, Burkina Faso.
⁴ Department of Molecular Biology, Pietro Annigoni Biomolecular Research Center (CERBA), Ouagadougou 01, Burkina Faso; Department of Laboratory of Molecular and Genetic Biology (LABIOGENE), Joseph KI-ZERBO University, Ouagadougou 03, Burkina Faso; Faculty of Medicine, Saint Thomas Aquinas University (USTA), Ouagadougou 06, Burkina Faso.
⁵ Department of Laboratory of Virology and Plant Biotechnology, Institute for the Environment and Agricultural Research (INERA/CNRST), Ouagadougou 01, Burkina Faso.

Abstract

Aim: Glaucoma is a group of degenerative diseases of the optic nerve whose predisposing factors may be genetic. The objective of this study was to estimate the frequency of the Glu323Lys mutation as a genetic risk factor for glaucoma.

Materials and methods: A cross-sectional study over 6 months from October 2020 to March 2021 in Ouagadougou, Burkina Faso. A total of 89 samples of patients with primary open-angle glaucoma (POAG) were collected. The frequency of the Glu323Lys mutation of the myocilin, trabecular meshwork inducible glucocorticoid response (TIGR/MYOC) gene by polymerase chain reaction (PCR)-restriction fragment length polymorphism.

Results: In glaucoma patients, only homozygous nonmutated guanine-guanine (GG) and heterozygous mutated adenine-guanine (AG) genotypes were found in 96.63 and 3.37% of cases, respectively. Around 69.66% of patients had a family history of glaucoma, 28.09% had a history of hypertension, and 7.86% had a history of diabetes.

Conclusion: The frequency of the Glu323Lys mutation of the TIGR/MYOC gene was 3.37% in the glaucoma population in Ouagadougou. A case-control study is necessary to know the contribution of the Glu323Lys mutation as a genetic risk factor for glaucoma in our study population.

Clinical significance: This study constituted the beginning of genetic investigations of glaucoma in our context and showed a low Glu323Lys mutation.

How to cite this article: Traoré L, Sanou J, Bakyono BS, et al. Prevalence of Glu323Lys Mutation of the TIGR/MYOC Gene and Risk Factors amongst Primary Open-angle Glaucoma Patients in Ouagadougou, Burkina Faso. J Curr Glaucoma Pract 2023;17(2):79-84.

Keywords: Burkina Faso; Glu323Lys mutation; Risk factors glaucoma.