Regulation of the WNK4-SPAK-NCC pathway by the calcium-sensing receptor

Curr Opin Nephrol Hypertens. 2023 Sep 1;32(5):451-457. doi: 10.1097/MNH.0000000000000915. Epub 2023 Jul 7.

Abstract

Purpose of review: Regulation of the sodium chloride cotransporter (NCC) in the distal convoluted tubule (DCT) plays a crucial role in renal salt handling. The calcium-sensing receptor (CaSR) has been shown to activate NCC through the WNK4-SPAK pathway, which is independent of the Renin-Angiotensin-Aldosterone system. In this review, we examine new information about the mechanism of how the CaSR regulates NCC through the WNK4-SPAK pathway and its physiological and therapeutic implications.

Recent findings: The activation of CaSR in TALH cells during hypercalcemia inhibits NKCC2 and ROMK activity, reducing paracellular Ca2+ reabsorption but decreasing salt reabsorption. This pathway enables NaCl reabsorption in the DCT while promoting Ca2+ excretion. CaSR activation in the apical DCT stimulates a signaling pathway involving PKC, WNK4, and SPAK, which increases NCC activation to recover the NaCl not reabsorbed in TAHL. Glucose or fructose acting as calcimimetics enhance apical CaSR sensitivity, increasing NCC activity, which contribute to the mechanism of hypertension prevalence in diabetic patients or in those with high fructose consumption.

Summary: These findings reveal the importance of the CaSR-mediated activation of the WNK4-SPAK pathway in regulating salt and calcium homeostasis and its potential as a therapeutic target for hypertension and related diseases.

Publication types

  • Review
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Calcium / metabolism
  • Humans
  • Hypertension* / metabolism
  • Kidney Tubules, Distal / metabolism
  • Phosphorylation
  • Protein Serine-Threonine Kinases* / metabolism
  • Receptors, Calcium-Sensing / metabolism
  • Sodium Chloride / metabolism
  • Sodium Chloride, Dietary / metabolism
  • Solute Carrier Family 12, Member 3 / metabolism

Substances

  • Protein Serine-Threonine Kinases
  • Receptors, Calcium-Sensing
  • Sodium Chloride
  • Calcium
  • Sodium Chloride, Dietary
  • Solute Carrier Family 12, Member 3
  • WNK4 protein, human