Type of intrinsic resistant starch type 3 determines in vitro fermentation by pooled adult faecal inoculum

C E Klostermann; M F Endika; E Ten Cate; P L Buwalda; P de Vos; J H Bitter; E G Zoetendal; H A Schols

doi:10.1016/j.carbpol.2023.121187

Type of intrinsic resistant starch type 3 determines in vitro fermentation by pooled adult faecal inoculum

Carbohydr Polym. 2023 Nov 1:319:121187. doi: 10.1016/j.carbpol.2023.121187. Epub 2023 Jul 8.

Authors

C E Klostermann¹, M F Endika², E Ten Cate¹, P L Buwalda³, P de Vos⁴, J H Bitter⁵, E G Zoetendal², H A Schols⁶

Affiliations

¹ Biobased Chemistry and Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; Laboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.
² Laboratory of Microbiology, Wageningen University & Research, Stippeneng 4, 6708 WE Wageningen, the Netherlands.
³ Biobased Chemistry and Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands; Coöperatie Koninklijke AVEBE u.a., P.O. Box 15, 9640 AA Veendam, the Netherlands.
⁴ Immunoendocrinology, Division of Medical Biology, Department of Pathology and Medical Biology, University of Groningen and University Medical Centre Groningen, Groningen, Hanzeplein 1, 9700 RB Groningen, the Netherlands.
⁵ Biobased Chemistry and Technology, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands.
⁶ Laboratory of Food Chemistry, Wageningen University & Research, Bornse Weilanden 9, 6708 WG Wageningen, the Netherlands. Electronic address: henk.schols@wur.nl.

PMID: 37567720
DOI: 10.1016/j.carbpol.2023.121187

Abstract

Resistant starch (RS) results in relatively high health-beneficial butyrate levels upon fermentation by gut microbiota. We studied how physico-chemical characteristics of RS-3 influenced butyrate production during fermentation. Six highly resistant RS-3 substrates (intrinsic RS-3, 80-95 % RS) differing in chain length (DPn 16-76), Mw distribution (PI) and crystal type (A/B) were fermented in vitro by pooled adult faecal inoculum. All intrinsic RS-3 substrates were fermented to relatively high butyrate levels (acetate/butyrate ≤ 2.5), and especially fermentation of A-type RS-3 prepared from polydisperse α-1,4 glucans resulted in the highest relative butyrate amount produced (acetate/butyrate: 1). Analysis of the microbiota composition after fermentation revealed that intrinsic RS-3 stimulated primarily Lachnospiraceae, Bifidobacterium and Ruminococcus, but the relative abundances of these taxa differed slightly depending on the RS-3 physico-chemical characteristics. Especially intrinsic RS-3 of narrow disperse Mw distribution stimulated relatively more Ruminococcus. Selected RS fractions (polydisperse Mw distribution) obtained after pre-digestion were fermented to acetate and butyrate (ratio ≤ 1.8) and stimulated Lachnospiraceae and Bifidobacterium. This study indicates that especially the α-1,4 glucan Mw distribution dependent microstructure of RS-3 influences butyrate production and microbiota composition during RS-3 fermentation.

Keywords: Butyrate; Crystal type; Dietary fibre; Microbiota; Molecular weight distribution.