Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence

Xiao Zhong; Ruochan Chen

doi:10.1007/978-1-0716-3433-2_19

Detection of Ferroptosis by Immunohistochemistry and Immunofluorescence

Methods Mol Biol. 2023:2712:211-222. doi: 10.1007/978-1-0716-3433-2_19.

Authors

Xiao Zhong^{1

2}, Ruochan Chen^{1

2}

Affiliations

¹ Department of Infectious Diseases, Xiangya Hospital, Central South University, Changsha, Hunan, China.
² Hunan Key Laboratory of Viral Hepatitis, Xiangya Hospital, Central South University, Changsha, Hunan, China.

PMID: 37578709
DOI: 10.1007/978-1-0716-3433-2_19

Abstract

Ferroptosis is a type of regulated cell death driven by oxidative damage, characterized by iron overload and lipid peroxidation, and regulated by a network of distinct molecules and organelles. Impaired ferroptotic response is implicated in multiple physiological and pathological processes, including tumorigenesis, neurodegeneration, and ischemia-reperfusion damage. Classical techniques of immunohistochemistry (IHC) and immunofluorescence (IF) can be employed to exhibit antigen expression and location in tissues observed with microscopy, making them powerful tools in studying the ferroptosis process. In this chapter, we introduce commonly used protocols and summarize typical markers used in IHC and IF to monitor ferroptosis.

Keywords: Ferroptosis; Immunofluorescence; Immunohistochemistry.

MeSH terms

Ferroptosis*
Fluorescent Antibody Technique
Immunohistochemistry
Lipid Peroxidation
Oxidative Stress