Our understanding of major developmental transitions in plants and animals has been transformed by the emergence of omics technologies. The majority of leaf growth research has been conducted at the transcriptional level. Although historically understudied, alterations at the protein and metabolite levels have begun to gain traction in recent years. Here, we present a protocol for metabolite and protein extraction followed by untargeted metabolomics and proteomics analysis of the growing leaves.
Keywords: Mass-spectrometry; Metabolomics; Methyl tertiary-butyl ether; Proteomics.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.