First report of L1 cell adhesion molecule (L1CAM) in flat fish (Starry flounder): Molecular features and expression analysis

Min-Young Sohn; Ju-Won Kim; Gyoungsik Kang; Won-Sik Woo; Kyung-Ho Kim; Ha-Jeong Son; Jong-Won Park; Do-Hyung Kim; Chan-Il Park

doi:10.1016/j.dci.2023.105054

First report of L1 cell adhesion molecule (L1CAM) in flat fish (Starry flounder): Molecular features and expression analysis

Dev Comp Immunol. 2023 Dec:149:105054. doi: 10.1016/j.dci.2023.105054. Epub 2023 Sep 9.

Authors

Min-Young Sohn¹, Ju-Won Kim², Gyoungsik Kang¹, Won-Sik Woo¹, Kyung-Ho Kim¹, Ha-Jeong Son¹, Jong-Won Park³, Do-Hyung Kim⁴, Chan-Il Park⁵

Affiliations

¹ Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong, 650-160, Republic of Korea.
² Biotechnology Research Division, National Institute of Fisheries Science, Busan, 46083, Republic of Korea.
³ Fish Breeding Research Center, Fisheries Seed Breeding Institute, National Institute of Fisheries Science, 81-9 Geojenamseo-ro, Nambu-myeon, Geoje, 53334, Republic of Korea.
⁴ Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University, 45, Yongso-ro, Nam-Gu., Busan, Republic of Korea. Electronic address: dhkim@pknu.ac.kr.
⁵ Department of Marine Biology & Aquaculture, College of Marine Science, Gyeongsang National University, 455, Tongyeong, 650-160, Republic of Korea. Electronic address: vinus96@hanmail.net.

PMID: 37690612
DOI: 10.1016/j.dci.2023.105054

Abstract

In this study, the starry flounder L1 cell adhesion molecule (L1CAM) sequence was obtained using next-generation sequencing, and the integrity of the sequence was verified by cloning and sequencing. First, the amino acid sequence was predicted using the cDNA sequence, and the gene was then identified through multiple sequence alignment analysis with related sequences and phylogenetic analysis. Thus, homogeneity was confirmed. The expression level of PsL1CAM (Platichthys stellatus L1CAM) mRNA in healthy starry flounder was detected in all tissues used in the experiment, and tissue- and gene-specific expression levels were confirmed. In addition, as a result of mRNA expression analysis after artificial infection with viral hemorrhagic septicemia virus (VHSV) and Streptococcus parauberis PH0710, significant expression changes and characteristics were confirmed following infection with VHSV and S. parauberis PH0710. After artificial infection with VHSV, the expression level of PsL1CAM mRNA was significantly upregulated in almost all major tissues of the starry flounder, whereas it was significantly downregulated in mucosal-associated lymphoid tissues, such as the gills and intestine. Infection with S. parauberis PH0710 significantly upregulated the expression of PsL1CAM mRNA in almost all major tissues of the starry flounder, whereas it was significantly downregulated in the heart after infection. Our results indicate that PsL1CAM may be involved in the host immune response to starry flounders.

Keywords: Immune response; L1CAM; Starry flounder; Streptococcus parauberis; Viral haemorrhagic septicemia virus.