Desire intolerance is conceptualized as a motivational counterpoint to the transdiagnostic risk factor of distress intolerance and is defined as the inability or unwillingness to "sit with" the motivation to approach a rewarding object or task. The current work describes the development and validation of a novel measure of desire intolerance. After initial item development and exploratory factor analysis (Study 1) and confirmatory factor analysis (Study 2), the 10-item unidimensional Desire Intolerance Questionnaire (DIQ) was created. Desire intolerance was conceptually related to low self-control (Studies 1-4) and both approach and avoidance motivation (Studies 2, 4, 5) and was distinct from delay of gratification (Study 3). Greater desire intolerance was also associated with higher distress intolerance (Studies 4 and 5). In Study 4, we used qualitative coding to explore what people were thinking about when they considered their "desires." Findings revealed that higher desire intolerance was associated with thinking about desired long-term outcomes more than proximal short-term hedonic pleasures. Finally, Study 4 focused on unique and independent associations between desire and distress intolerance with psychopathology, and we found that both distress intolerance were associated with heightened psychological distress and borderline personality symptoms. Only distress intolerance was uniquely associated with uncontrolled eating and self-criticism, whereas only desire intolerance was uniquely associated with aggressive behavior and dichotomous thinking. The current research supports the DIQ as a psychometrically sound indicator of difficulties withstanding desires and allows for a better understanding of intolerance as a transdiagnostic clinical phenomena. (PsycInfo Database Record (c) 2023 APA, all rights reserved).