By using the Api-Zym System 55 enzymes were determined to be present in bee venom, venom sac, sacless whole body extract and whole body commercial extracts. Acid phosphatase activity was high in bee venom and sacless whole body extracts. Arylamidase activities were elevated in sacless whole body extracts and very low or undetectable in venoms. Conversely, glycyl-prolin arylamidase activity was high in venoms and very low in the other extracts.