Interleukin-6 and -27 as potential novel biomarkers for human pleural tuberculosis regardless of the immunological status

Paulo Antas; Juliana Borchert; Carlos Ponte; Jessica Lima; Ingebourg Georg; Mayara Bastos; Anete Trajman

doi:10.1016/j.micinf.2023.105238

Interleukin-6 and -27 as potential novel biomarkers for human pleural tuberculosis regardless of the immunological status

Microbes Infect. 2024 Jan-Feb;26(1-2):105238. doi: 10.1016/j.micinf.2023.105238. Epub 2023 Oct 5.

Authors

Paulo Antas¹, Juliana Borchert², Carlos Ponte², Jessica Lima², Ingebourg Georg³, Mayara Bastos⁴, Anete Trajman⁵

Affiliations

¹ Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil. Electronic address: paulo.zuquim@fiocruz.br.
² Oswaldo Cruz Institute, FIOCRUZ, Rio de Janeiro, Brazil.
³ Evandro Chagas National Institute of Infectious Diseases, FIOCRUZ, Rio de Janeiro, Brazil.
⁴ McGill University, Montreal, Canada.
⁵ McGill University, Montreal, Canada; Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.

PMID: 37805123
DOI: 10.1016/j.micinf.2023.105238

Abstract

Tuberculosis (TB) is the leading cause of pleural exudative effusions. Inflammatory markers, such as IFNγ and ADA, have been used as proxies for its diagnosis. We evaluated ex vivo levels of several cytokines in 83 pleural effusion specimens from patients with TB (including 10 with HIV co-infection) and 26 patients with other pleuritis using multiplex and ELISA assays. IL-6 and IL-27 levels were higher (p ≤ 0.04) in TB patients, regardless of the HIV status and the approach. IL-2, IL-4, IL-8, IFNγ, TNF and G-CSF showed variable results depending on the assay. This warranty these markers to be further validated.

Keywords: Cytokine; HIV; Human; Pleural; Tuberculosis.

MeSH terms

Biomarkers / analysis
Cytokines
HIV Infections* / complications
Humans
Interleukin-6
Pleural Effusion* / diagnosis
Pleural Effusion* / etiology
Tuberculosis, Pleural* / complications
Tuberculosis, Pleural* / diagnosis

Substances

Interleukin-6
Cytokines
Biomarkers