A Semi-Automatic Environmental Monitoring Device for Mercury and Cobalt Ion Detection

Yi-Ru Chiou; Hao-Ming Pang; Yu-Fen Huang; Chien-Fu Chen

doi:10.1002/smll.202303871

A Semi-Automatic Environmental Monitoring Device for Mercury and Cobalt Ion Detection

Small. 2024 Feb;20(8):e2303871. doi: 10.1002/smll.202303871. Epub 2023 Oct 10.

Authors

Yi-Ru Chiou^{1

2

3}, Hao-Ming Pang¹, Yu-Fen Huang^{3

4}, Chien-Fu Chen^{1

2}

Affiliations

¹ Institute of Applied Mechanics, National Taiwan University, Taipei, 106, Taiwan.
² Graduate School of Advanced Technology, National Taiwan University, 106, Taipei, Taiwan.
³ Institute of Analytical and Environmental Sciences, National Tsing Hua University, Hsinchu, 300, Taiwan.
⁴ Department of Biomedical Engineering and Environmental Sciences, National Tsing Hua University, Hsinchu, 300, Taiwan.

PMID: 37817349
DOI: 10.1002/smll.202303871

Abstract

A syringe-based, semi-automatic environmental monitoring device is developed for on-site detection of harmful heavy metal ions in water. This portable device consists of a spring-embedded syringe and a polydimethylsiloxane (PDMS) membrane-based flow regulator for semi-automatic fix-and-release fluidic valve actuation, and a paper-based analytical device (PAD) with two kinds of gold nanoclusters (AuNCs) for sensitive Hg²⁺ and Co²⁺ ion detection, respectively. The thickness of the elastic PDMS membrane can be adjusted to stabilize and modulate the flow rates generated by the pushing force provided by the spring attached to the plunger. Also, different spring constants can drastically alter the response time. People of all ages can extract the fix-volume sample solutions and then release them to automatically complete the detection process, ensuring high reliability and repeatability. The PAD comprises two layers of modified paper, and each layer is immobilized with bovine serum albumin-capped gold nanoclusters (R-AuNCs) and glutathione-capped gold clusters (G-AuNCs), respectively. The ligands functionalized on the surface of the AuNCs not only can fine-tune the optical properties of the nanoclusters but also enable specific and simultaneous detection of Hg²⁺ and Co²⁺ ions via metallophilic Au⁺ -Hg²⁺ interaction and the Co²⁺ -thiol complexation effect, respectively. The feasibility of the device for detecting heavy metal ions at low concentrations in various environmental water samples is demonstrated. The Hg²⁺ and Co²⁺ ions can be seen simultaneously within 20 min with detection limits as low as 1.76 nm and 0.27 µm, respectively, lower than those of the regulatory restrictions on water by the US Environmental Protection Agency and the European Union. we expect this sensitive, selective, portable, and easy-to-use device to be valid for on-site multiple heavy metal ion pollution screenings in resource-constrained settings.

Keywords: gold nanoclusters; heavy metals detection; paper-based analytical devices; syringe-based devices.

Abstract

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