A Novel Sandwich Method for Serial Block Face SEM Imaging of Airway Multiciliated Epithelium

Methods Mol Biol. 2024:2725:121-129. doi: 10.1007/978-1-0716-3507-0_7.

Abstract

Volume electron microscopy technologies such as serial block face scanning electron microscopy (SBF-SEM) allow the characterization of tissue organization and cellular content in three dimensions at nanoscale resolution. Here, we describe the procedure to process and image an air-liquid interface culture of human or mouse airway epithelial cells for visualization of the multiciliated epithelium by SBF-SEM in vertical or horizontal cross section.

Keywords: Air-liquid interface cultures; Airway epithelial cells; Sandwich embedding; Serial block face scanning electron microscopy; VolumeEM.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Epithelial Cells
  • Epithelium
  • Humans
  • Imaging, Three-Dimensional* / methods
  • Mice
  • Microscopy, Electron, Scanning
  • Volume Electron Microscopy*